Protein and biologics characterisation

HPLC autosampler

Once your protein or biologics have been purified, the RPF team is ready to carry out its characterisation.

 

Quality Attributes:

The Recombinant Products Facility offers access to a comprehensive suite of instruments and expertise in various techniques to determine key attributes of your protein or biologics, including:

  • Identity
  • Size
  • Mass
  • Oligomeric state
  • Purity
  • Concentration
  • Stability

Additionally, we can perform assays to detect and quantify specific contaminants such as:

  • Host Cell DNA
  • Host Cell Protein
  • Endotoxins

Our team is dedicated to providing thorough and precise characterisation to meet your needs.

Analytical capabilities:

At the Recombinant Products Facility, we routinely perform a range of analytical assays, including:

  • SDS-PAGE
  • Western Blot
  • ELISA
  • BCA (Bicinchoninic Acid) Assay
  • Bradford Assay
  • Qubit
  • UV-Vis Spectroscopy
  • qPCR (Quantitative PCR)

For more specific characterisation tests, the RPF also has access to:

  • HPLC (bioinert)

At the Recombinant Products Facility, we come across very different projects requiring the use of a large variety of HPLC columns and methods.

Our Bioinert HPLC with its temperature regulated autosampler (from 4 to 40°C), DAD fluorescence detector and fraction collector in combination with our wide range of HPLC columns available is a great asset to analyse your protein; for example to determine protein homogenity (charged variants, glycosylation) or aggregation.

We have a selection of Size exclusion, anion, cation exchange as well as Reverse Phase columns (list available on demand). Some clients prefer to bring their own specific columns, and we develop for them a method for their own characterisation.

 

  • Prometheus (nanotemper)

The Prometheus NT48 allows the measurement of protein unfolding and aggregation.

Buffer formulations can be screened to find the optimal buffer of your final product.

This instrument is as well used as quality control for:

-Long-term stability of biologics

-Forced degradation

-Thermal stability of antibody/Drug conjugates

and has a specific software developed for stability studies.

 

  • Mass Photometer (refeyn)

The Mass Photometer allows accurate mass measurement of single molecules in solution, in their native state and without the need for labels giving information about sample homogeneity, structural integrity with minimal sample amount.

 

UNSW hosts other accessible facilities (see MWAC) Listed below are those related to proteins/biologics.

protein characterisation, using the BioInert HPLC and the Prometheus NanoDSF